combination with gcv Search Results


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InvivoGen combination with gcv
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JHP Pharmaceuticals ganciclovir
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JHP Pharmaceuticals ganciclovir gcv
Gene transduction efficacy of adenovirus (Ad)5/35 is enhanced in CD46-expressing cells. ( A ) Western blot analysis of CAR and CD46 expression in parental rodent baby hamster kidney (BHK) cells or BHK-CAR and BHK-CD46 cells, which ectopically express the Ad receptors CAR and CD46, respectively. ( B ) Flow cytometry analysis of Ad-mediated green fluorescent protein (GFP) expression in BHK, BHK-CAR, and BHK-CD46 cells. Transduction of GFP by Ad5 (left panel) or Ad5/35 (right panel) analysis was measured by flow cytometry. ( C ) A cell killing assay was performed for all three BHK cell lines using either Ad5-tk (left panel) or Ad5/35-tk (right panel) followed by <t>ganciclovir</t> (GCV) treatment. Cytotoxicity was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Error bars represent standard error (SEM). Statistics: C, p < 0.01 by two-way analysis of variance (ANOVA). Confirmed.
Ganciclovir Gcv, supplied by JHP Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Genlantis inc gcv
Gene transduction efficacy of adenovirus (Ad)5/35 is enhanced in CD46-expressing cells. ( A ) Western blot analysis of CAR and CD46 expression in parental rodent baby hamster kidney (BHK) cells or BHK-CAR and BHK-CD46 cells, which ectopically express the Ad receptors CAR and CD46, respectively. ( B ) Flow cytometry analysis of Ad-mediated green fluorescent protein (GFP) expression in BHK, BHK-CAR, and BHK-CD46 cells. Transduction of GFP by Ad5 (left panel) or Ad5/35 (right panel) analysis was measured by flow cytometry. ( C ) A cell killing assay was performed for all three BHK cell lines using either Ad5-tk (left panel) or Ad5/35-tk (right panel) followed by <t>ganciclovir</t> (GCV) treatment. Cytotoxicity was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Error bars represent standard error (SEM). Statistics: C, p < 0.01 by two-way analysis of variance (ANOVA). Confirmed.
Gcv, supplied by Genlantis inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ViroStatics SRL gcv
Gene transduction efficacy of adenovirus (Ad)5/35 is enhanced in CD46-expressing cells. ( A ) Western blot analysis of CAR and CD46 expression in parental rodent baby hamster kidney (BHK) cells or BHK-CAR and BHK-CD46 cells, which ectopically express the Ad receptors CAR and CD46, respectively. ( B ) Flow cytometry analysis of Ad-mediated green fluorescent protein (GFP) expression in BHK, BHK-CAR, and BHK-CD46 cells. Transduction of GFP by Ad5 (left panel) or Ad5/35 (right panel) analysis was measured by flow cytometry. ( C ) A cell killing assay was performed for all three BHK cell lines using either Ad5-tk (left panel) or Ad5/35-tk (right panel) followed by <t>ganciclovir</t> (GCV) treatment. Cytotoxicity was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Error bars represent standard error (SEM). Statistics: C, p < 0.01 by two-way analysis of variance (ANOVA). Confirmed.
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Image Search Results


Gene transduction efficacy of adenovirus (Ad)5/35 is enhanced in CD46-expressing cells. ( A ) Western blot analysis of CAR and CD46 expression in parental rodent baby hamster kidney (BHK) cells or BHK-CAR and BHK-CD46 cells, which ectopically express the Ad receptors CAR and CD46, respectively. ( B ) Flow cytometry analysis of Ad-mediated green fluorescent protein (GFP) expression in BHK, BHK-CAR, and BHK-CD46 cells. Transduction of GFP by Ad5 (left panel) or Ad5/35 (right panel) analysis was measured by flow cytometry. ( C ) A cell killing assay was performed for all three BHK cell lines using either Ad5-tk (left panel) or Ad5/35-tk (right panel) followed by ganciclovir (GCV) treatment. Cytotoxicity was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Error bars represent standard error (SEM). Statistics: C, p < 0.01 by two-way analysis of variance (ANOVA). Confirmed.

Journal: International Journal of Molecular Sciences

Article Title: Targeting CD46 Enhances Anti-Tumoral Activity of Adenovirus Type 5 for Bladder Cancer

doi: 10.3390/ijms19092694

Figure Lengend Snippet: Gene transduction efficacy of adenovirus (Ad)5/35 is enhanced in CD46-expressing cells. ( A ) Western blot analysis of CAR and CD46 expression in parental rodent baby hamster kidney (BHK) cells or BHK-CAR and BHK-CD46 cells, which ectopically express the Ad receptors CAR and CD46, respectively. ( B ) Flow cytometry analysis of Ad-mediated green fluorescent protein (GFP) expression in BHK, BHK-CAR, and BHK-CD46 cells. Transduction of GFP by Ad5 (left panel) or Ad5/35 (right panel) analysis was measured by flow cytometry. ( C ) A cell killing assay was performed for all three BHK cell lines using either Ad5-tk (left panel) or Ad5/35-tk (right panel) followed by ganciclovir (GCV) treatment. Cytotoxicity was analyzed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Error bars represent standard error (SEM). Statistics: C, p < 0.01 by two-way analysis of variance (ANOVA). Confirmed.

Article Snippet: To evaluate the in vitro cell killing efficacy of thymidine kinase-expressing viruses (Ad5-tk and Ad5/35-tk) in combination with ganciclovir (GCV, JHP Pharmaceuticals LLC., Rochester, MI, USA), 1 × 10 4 cells per well were seeded into 24-well plates.

Techniques: Transduction, Expressing, Western Blot, Flow Cytometry, MTT Assay